RT Journal Article
SR Electronic
T1 An Arrayed Transposon Library of <em>Ruegeria pomeroyi</em> DSS-3
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.09.11.507510
DO 10.1101/2022.09.11.507510
A1 Mejia, Catalina
A1 Trujillo Rodriguez, Lidimarie
A1 Poudel, Ravin
A1 Ellington, Adam
A1 Rivers, Adam R.
A1 Reisch, Christopher R.
YR 2022
UL http://biorxiv.org/content/early/2022/09/14/2022.09.11.507510.abstract
AB The ability to construct defined genetic mutations in many bacteria is difficult and limited. Transposon mutagenesis is often highly efficient, but is not site specific, thus selections are often needed to identify mutants of interest. The construction of arrayed mutant libraries would help to fill this need, though these libraries are costly and time consuming. To enable easier construction of arrayed libraries we developed a workflow and methodology using a hierarchical barcoding scheme to identify mutants within a multiwell plate. We applied this method to the marine Alphaproteobacterium Ruegeria pomeroyi DSS-3 and created a library with over 2,800 disrupted genes.Competing Interest StatementThe authors have declared no competing interest.