%0 Journal Article %A Fabio Manca %A Gautier Eich %A Omar N’Dao %A Lucie Normand %A Kheya Sengupta %A Laurent Limozin %A Pierre-Henri Puech %T Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction %D 2022 %R 10.1101/2022.09.15.508080 %J bioRxiv %P 2022.09.15.508080 %X The role of force application in immune cell recognition is now well established, the force being transmitted between the actin cytoskeleton to the anchoring ligands through receptors such as integrins. In this chain, the mechanics of the cytoskeleton to receptor link, though clearly crucial, remains poorly understood. To probe this link, we combine mechanical extraction of membrane tubes from T cells using optical tweezers, and fitting of the resulting force curves with a viscoelastic model taking into account the cell and relevant molecules. We find that it is possible to separate the cell-scale and molecular-scale effects and thus to estimate the stiffness of this putative link to be of the order of 0.05 pN nm−1. We solicit this link using four different antibodies against various membrane bound receptors: antiCD3 to target the T Cell Receptor (TCR) complex, antiCD45 for the long sugar CD45, and two clones of antiCD11 targeting open or closed conformation of LFA1 integrins. Upon disruption of the cytoskeleton, the stiffness of the link changes for two of the receptors, exposing the existence of a receptor to cytoskeleton link - namely TCR-complex and open LFA1, and does not change for the other two where no such a link was expected. Our integrated approach allows us to probe, for the first time, the mechanics of the intracellular receptor-cytoskeleton link in immune cells. In doing so, we provide a quantitative value for the elasticity of the putative link, and elucidate a mechanism bridging molecular and cellular scales.Significance Statement T cells are the first responders of the adaptive immune system via the recognition of a non-self peptide via the T cell receptor (TCR). Generating force by actomyosin machinery, T cells test the TCR to antigen binding. This force needs to be transmitted via a chain of molecules where the receptor-to-cytoskeleton interaction is currently the missing link. We devised a method based on pulling membrane tubes via optical tweezers and a physical model to extract vis-coelastic parameters, separable into cell or molecular scales, to probe the mechanics of the receptor to cytoskeleton link. Our analysis suggests that the link stiffness depends on the identity of the receptor being sollicitated. These findings have implications for understanding of T cell mechanotransduction.Competing Interest StatementThe authors have declared no competing interest. %U https://www.biorxiv.org/content/biorxiv/early/2022/09/15/2022.09.15.508080.full.pdf