@article {Watkins-Chow097220, author = {Dawn E. Watkins-Chow and Gaurav K. Varshney and Lisa J. Garrett and Zelin Chen and Erin A. Jimenez and Cecilia Rivas and Kevin S. Bishop and Raman Sood and Ursula L. Harper and William J. Pavan and Shawn M. Burgess}, title = {Highly-efficient Cpf1-mediated gene targeting in mice following high concentration pronuclear injection}, elocation-id = {097220}, year = {2016}, doi = {10.1101/097220}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Cpf1 has emerged as an alternative to the Cas9 RNA-guided nuclease. Here we show that gene targeting rates in mice using Cpf1 can meet or even surpass Cas9 targeting rates (approaching 100\% targeting) but require higher concentrations of mRNA and guide. We also demonstrate that co-injecting two guides with close targeting sites can result in synergistic genomic cutting, even if one of the guides has minimal cutting activity.}, URL = {https://www.biorxiv.org/content/early/2016/12/28/097220}, eprint = {https://www.biorxiv.org/content/early/2016/12/28/097220.full.pdf}, journal = {bioRxiv} }