RT Journal Article SR Electronic T1 Whole-genome sequencing and single acute toxicity of heavy metal to Photobacterium kishitanii FJ21 JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.09.20.508755 DO 10.1101/2022.09.20.508755 A1 Shuzheng Yin A1 Zilong Yi A1 Jia Liu A1 Gang Liu A1 Jun Fang YR 2022 UL http://biorxiv.org/content/early/2022/09/21/2022.09.20.508755.abstract AB In this study, the growth morphology of FJ21 strain was observed, and its 16S rRNA and whole genome were sequenced. Then, related software was used to make genome assembly, gene structure and function annotation, genome phylogenetic tree analysis, genome collinearity analysis and prediction of secondary metabolic gene cluster analysis. Finally, the single acute toxicity of five heavy metals to FJ21 strain was detected. There were luxC, luxD, luxA, luxB, luxF, luxE and luxG genes in FJ21, and the protein encoded by lux operon had certain hydrophilicity. The genome of this strain FJ21 contains a chromosome with a total length of 4853277bp and a GC content of 39.23%. The genome of FJ21 was compared with that of Photobacterium kishitanii ATCCBAA-1194, Photobacterium phosphoreum JCM21184, Photobacterium aquimaris LC2-065, Photobacterium malacitanum CECT9190, and Photobacterium carnosum TMW 2.2021. The average nucleotide identity(ANI), tetra nucleotide signatures (Tetra), comparative genome, and phylogenetic analysis proposed that FJ21 is a strain of Photobacterium kishitanii. In the acute toxicity test, the toxicity of heavy metals to the strain FJ21 is Pb(NO3)2 > ZnSO4·7H2O > CdCl2·2.5H2O > CuSO4·5H2O > K2Cr2O7.