PT  - JOURNAL ARTICLE
AU  - Christian S. Stevens
AU  - Jake Lowry
AU  - Terry Juelich
AU  - Colm Atkins
AU  - Kendra Johnson
AU  - Jennifer K. Smith
AU  - Maryline Panis
AU  - Tetsuro Ikegami
AU  - Benjamin tenOever
AU  - Alexander N. Freiberg
AU  - Benhur Lee
TI  - Nipah virus Bangladesh infection elicits organ-specific innate and inflammatory responses in the marmoset model
AID  - 10.1101/2021.10.11.463955
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2021.10.11.463955
4099  - http://biorxiv.org/content/early/2022/09/22/2021.10.11.463955.short
4100  - http://biorxiv.org/content/early/2022/09/22/2021.10.11.463955.full
AB  - The common marmoset (Callithrix jacchus) is increasingly recognized as an ideal non-human primate (NHP) at high-biocontainment due to its smaller size and relative ease of handling. Here, we evaluated the susceptibility and pathogenesis of Nipah virus Bangladesh strain (NiVB) infection in marmosets at biosafety level 4. Infection via the intranasal and intratracheal route resulted in fatal disease in all four infected marmosets. Three developed pulmonary edema and hemorrhage as well as multi-focal hemorrhagic lymphadenopathy, while one recapitulated neurologic clinical symptoms and cardiomyopathy on gross pathology. Organ-specific innate and inflammatory responses were characterized by RNA-seq in six different tissues from infected and control marmosets. Notably, a unique transcriptome was revealed in the brainstem of the marmoset exhibiting neurological symptoms. Our results provide a more comprehensive understanding of NiV pathogenesis in an accessible and novel NHP model, closely reflecting clinical disease as observed in NiV patients.Competing Interest StatementThe authors have declared no competing interest.