RT Journal Article
SR Electronic
T1 Genome characterization of monkeypox cases detected in India: Identification of three sub clusters among A.2 lineage
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.09.16.507742
DO 10.1101/2022.09.16.507742
A1 Anita M. Shete
A1 Pragya D. Yadav
A1 Abhinendra Kumar
A1 Savita Patil
A1 Deepak Y. Patil
A1 Yash Joshi
A1 Triparna Majumdar
A1 Vineet Relhan
A1 Rima R. Sahay
A1 Meenakshy Vasu
A1 Pranita Gawande
A1 Ajay Verma
A1 Arbind Kumar
A1 Shivram Dhakad
A1 Anukumar Bala Krishnan
A1 Shubin Chenayil
A1 Suresh Kumar
A1 Priya Abraham
YR 2022
UL http://biorxiv.org/content/early/2022/09/22/2022.09.16.507742.abstract
AB Since May 2022, Monkeypox, a zoonotic Orthopox DNA virus was reported in more than 102 countries indicating expansion of its geographic range. We analyzed the complete genomes sequences of Monkeypox cases from Kerala (n=5 travelled from UAE) and Delhi (n=5 no travel history), India confirmed during July to August 2022. All the retrieved MPXV sequences from India covering 90 to 99% genome belong to A.2 lineage of clade IIb. The A.2 MPXV lineage divided in three sub clusters; first cluster Kerala n=5, Delhi n=2 aligned with the USA-2022 ON674051.1; while second of Delhi n=3 aligned with USA-2022 ON675438.1 and third consists of the UK, USA and Thailand. Recent update in MPXV lineage designated all the five sequences from Kerala as A.2.1. In addition to known 16 single nucleotide polymorphisms (SNPs) along with 13 APOBEC3 cytosine deaminase activity determined specific lineage defining mutations in A.2 lineage, 25 additional APOBEC3 mutations were found in 10 reported sequences. The study emphasizes need of enhancing genomic surveillance to understand the mutation and its linkage.Competing Interest StatementThe authors have declared no competing interest.