RT Journal Article
SR Electronic
T1 Cohesin mediates DNA loop extrusion and sister chromatid cohesion by distinct mechanisms
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.09.23.509019
DO 10.1101/2022.09.23.509019
A1 Nagasaka, Kota
A1 Davidson, Iain F.
A1 Stocsits, Roman R.
A1 Tang, Wen
A1 Wutz, Gordana
A1 Betty, Paul
A1 Panarotto, Melanie
A1 Litos, Gabriele
A1 Schleiffer, Alexander
A1 Gerlich, Daniel Wolfram
A1 Peters, Jan-Michael
YR 2022
UL http://biorxiv.org/content/early/2022/09/23/2022.09.23.509019.abstract
AB Cohesin connects CTCF binding sites and other genomic loci in cis to form chromatin loops, and replicated DNA molecules in trans to mediate sister chromatid cohesion. Whether cohesin uses distinct or related mechanisms to perform these functions is unknown. Here we describe a cohesin hinge mutant, which can extrude DNA into loops but is unable to mediate cohesion. Our results suggest that the latter defect arises during cohesion establishment. The observation that cohesin’s cohesion and loop extrusion activities can be separated indicates that cohesin uses distinct mechanisms to perform these two functions. Unexpectedly, the same hinge mutant can also not be stopped by CTCF boundaries as well as wildtype cohesin. This suggests that cohesion establishment and cohesin’s interaction with CTCF boundaries depend on related mechanisms and raises the possibility that both require transient hinge opening to entrap DNA inside the cohesin ring.Competing Interest StatementThe authors have declared no competing interest.