RT Journal Article
SR Electronic
T1 grID: A CRISPR-Cas9 guide RNA Database and Resource for Genome-Editing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 097352
DO 10.1101/097352
A1 Vinod Jaskula-Ranga
A1 Donald J. Zack
YR 2016
UL http://biorxiv.org/content/early/2016/12/30/097352.abstract
AB CRISPR-Cas9 genome-editing is a revolutionary technology that is transforming biological research. The explosive growth and advances in CRISPR research over the last few years, coupled with the potential for clinical applications and therapeutics, is heralding a new era for genome engineering. To further support this technology platform and to provide a universal CRISPR annotation system, we introduce the grID database (http://crispr.technology), an extensive compilation of gRNA properties including sequence and variations, thermodynamic parameters, off-target analyses, and alternative PAM sites, among others. To aid in the design of optimal gRNAs, the website is integrated with other prominent databases, providing a wealth of additional resources to guide users from in silico analysis through experimental CRISPR targeting. Here, we make available all the tools, protocols, and plasmids that are needed for successful CRISPR-based genome targeting.