RT Journal Article
SR Electronic
T1 A Murine Model of Abductor Insufficiency Accelerates the Development of Hip Osteoarthritis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.05.22.492940
DO 10.1101/2022.05.22.492940
A1 Michael B. Geary
A1 Caitlin A. Orner
A1 Helen Shammas
A1 John M. Reuter
A1 Alayna E. Loiselle
A1 Chia-Lung Wu
A1 Brian D. Giordano
YR 2022
UL http://biorxiv.org/content/early/2022/09/25/2022.05.22.492940.abstract
AB Osteoarthritis (OA) of the hip is a common and debilitating painful joint disease. A growing body of evidence suggests that there may be an association between periarticular myotendinous pathology and the development of hip OA. Thus, we hypothesized that a murine model of hip OA could be achieved through selective injury of the abductor complex around the hip. C57BL6/J mice were randomized to sham surgery or abductor injury, in which the myotendinous insertion at the third trochanter and greater trochanter were surgically detached. Mice were allowed free, active movement until sacrifice at either 3 weeks or 20 weeks post-injury. Histologic analyses and immunohistochemical staining (IHC) of the femoral head articular cartilage were performed, along with μCT analysis to assess subchondral bone remodeling. We observed that mice receiving abductor injury exhibited significant OA severity with loss of Type II Collagen staining compared to sham control mice at 20 weeks post-surgery, comparable MMPI13 expression was observed between injury and sham groups. No significant differences in subchondral bone were found on μCT after 20 weeks following injury. Our study suggests a link between abductor dysfunction and the development of hip OA, which are common pathomorphologies encountered in routine orthopaedic clinical practice. Further, this novel animal model may provide a valuable tool for future investigations into the pathogenesis and treatment of hip OA.Competing Interest StatementThe authors have declared no competing interest.