RT Journal Article SR Electronic T1 3D Ground Truth Annotations of Nuclei in 3D Microscopy Volumes JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.09.26.509542 DO 10.1101/2022.09.26.509542 A1 Alain Chen A1 Liming Wu A1 Seth Winfree A1 Kenneth W. Dunn A1 Paul Salama A1 Edward J. Delp YR 2022 UL http://biorxiv.org/content/early/2022/09/26/2022.09.26.509542.abstract AB In this paper we describe a set of 3D microscopy volumes we have partially manually annotated. We describe the volumes annotated and the tools and processes we use to annotate the volumes. In addition, we provide examples of annotated subvolumes. We also provide synthetically generated 3D microscopy volumes that can be used for training segmentation methods. The full set of annotations, synthetically generated volumes, and original volumes can be accessed as described in the paper.Competing Interest StatementThe authors have declared no competing interest.