TY - JOUR T1 - The prophylactic value of TNF-α inhibitors against retinal ganglion cell and optic nerve axon loss after corneal surgery or trauma JF - bioRxiv DO - 10.1101/2022.10.06.510713 SP - 2022.10.06.510713 AU - Eleftherios I. Paschalis AU - Chengxin Zhou AU - Jyoti Sharma AU - Sarah Kim AU - Fengyang Lei AU - James Chodosh AU - Demetrios Vavvas AU - Arto Urtti AU - George Papaliodis AU - Claes H. Dohlman Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/10/08/2022.10.06.510713.1.abstract N2 - Background and Purpose: Late secondary glaucoma is an often severe complication after anterior segment surgery, trauma, infection, etc. TNF-α is a major mediator that is rapidly upregulated and causes retinal cell apoptosis and optic nerve axon degeneration (mediating steps to glaucomatous damage). Anti-TNF-α antibodies are in animals very effective in protecting the ganglion cells and the optic nerve—and might therefore be useful prophylactically against secondary glaucoma in patients. Here we evaluate 1) toxicity and 2) efficacy of two TNF-α inhibitors (adalimumab and infliximab), in rabbits by subconjunctival administration.Methods: For drug toxicity, animals with normal, unburned corneas were injected with adalimumab (0.4, 4, or 40 mg), or infliximab (1, 10, or 100 mg). For drug efficacy, other animals were subjected to alkali burn before such injection, or steroids. The rabbits were evaluated clinically with slit lamp and photography, electroretinography, optical coherence tomography, and intraocular pressure manometry. Some eyes were stained ex vivo after 3 days for retinal ganglion cell apoptosis (TUNEL). In other experiments the optic nerves were evaluated with paraphenylenediamine staining after 50 or 90 days. Loss of retinal ganglion cells and optic nerve degeneration were quantified.Results: Subconjunctival administration of 0.4 mg or 4.0 mg adalimumab were well tolerated, whereas 40.0 mg was toxic to the retina. 1, 10, or 100 mg infliximab were also well tolerated. Analysis of the optic nerve axons after 50 days confirmed the safety of 4.0 mg adalimumab or of 100 mg infliximab.For efficacy, 4.0 mg adalimumab subconjunctivally in 0.08 mL provided practically full protection against ganglion cell apoptosis 3 days following alkali burn, and infliximab 100 mg only slightly less. At 90 days following a burn, the control optic nerve showed about 50% axon loss but only about 8% if protected with adalimumab.Conclusions: Subconjunctival injection of 4.0 mg adalimumab in rabbits shows no eye toxicity and provides excellent neuroprotection, short (3 days) and long-term (90 days). Our total accumulated data from several studies,z combined with the present paper, suggest that corneal injuries, including surgery, might benefit from routine administration of anti-TNF-α biologics to reduce inflammation and future secondary glaucoma.Competing Interest StatementThe authors have declared no competing interest. ER -