RT Journal Article
SR Electronic
T1 Versatile and automated workflow for the analysis of oligodendroglial calcium signals in preclinical mouse models of myelin repair
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.10.14.512256
DO 10.1101/2022.10.14.512256
A1 Dorien A. Maas
A1 Blandine Manot-Saillet
A1 Philippe Bun
A1 Chloé Habermacher
A1 Corinne Poilbout
A1 Filippo Rusconi
A1 Maria Cecilia Angulo
YR 2022
UL http://biorxiv.org/content/early/2022/10/18/2022.10.14.512256.abstract
AB Intracellular Ca2+ signals of oligodendroglia, the myelin-forming cells of the central nervous system, regulate vital cellular processes including myelination. However, studies on oligodendroglia Ca2+ signal dynamics are still scarce, especially during myelin repair, and there are no software solutions to properly analyze the unique Ca2+ signal characteristics in these cells. Here, we provide a comprehensive experimental and analytical workflow to acquire and analyze Ca2+ imaging data of oligodendroglia at the population and single-cell levels in preclinical mouse models of myelin repair. We report diverse ex vivo and in vivo experimental protocols to obtain reproducible Ca2+ imaging data from oligodendroglia in demyelinated lesions. Importantly, we provide an analytical pipeline containing two free, open source and cross-platform software programs, Occam and post-prOccam, that enable the fully automated analysis of one- and two-photon Ca2+ imaging datasets from oligodendroglia obtained by either ex vivo or in vivo Ca2+ imaging techniques. This versatile and accessible experimental and analytical framework, which revealed significant but uncorrelated spontaneous Ca2+ activity in oligodendroglia inside demyelinated lesions, should facilitate the elucidation of Ca2+-mediated mechanisms underlying remyelination and therefore help to accelerate the development of therapeutic strategies for the many myelin-related disorders, such as multiple sclerosis.Competing Interest StatementThe authors have declared no competing interest.