RT Journal Article
SR Electronic
T1 Patterning of phase-separated condensates by Dnd1 controls cell fate
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.10.20.512863
DO 10.1101/2022.10.20.512863
A1 Kim Joana Westerich
A1 Katsiaryna Tarbashevich
A1 Antra Gupta
A1 Mingzhao Zhu
A1 Kenneth Hull
A1 Daniel Romo
A1 Theresa Gross-Thebing
A1 Erez Raz
YR 2022
UL http://biorxiv.org/content/early/2022/10/20/2022.10.20.512863.abstract
AB Germ granules, condensates of phase-separated RNA and protein, are essential for germline development, but how these molecules are organized within the granules and whether such an organization is relevant for germ cell fate is unclear. Combining three-dimensional in vivo structural and functional analyses, we study the dynamic spatial organization of molecules within zebrafish germ granules. We find that the vertebrate-specific Dead end protein is essential for positioning nanos3 RNA at the condensates’ periphery, where ribosomes are located. Without Dead end, or when translation is inhibited, nanos3 RNA translocates into granule interiors, far from the ribosomes’ location. These findings reveal the molecular mechanisms controlling the spatial organization of RNA within the phase-separated organelle and the importance of subgranule RNA localization for preserving germ cell totipotency.Competing Interest StatementThe authors have declared no competing interest.