RT Journal Article
SR Electronic
T1 Identification of two novel heterodimeric ABC transporters in melanoma: ABCB5β/B6 and ABCB5β/B9
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.10.21.513191
DO 10.1101/2022.10.21.513191
A1 Louise Gerard
A1 Laurent Duvivier
A1 Marie Fourrez
A1 Lindsay Sprimont
A1 Michael Gottesman
A1 Jean-Pierre Gillet
YR 2022
UL http://biorxiv.org/content/early/2022/10/21/2022.10.21.513191.abstract
AB ABCB5 is a member of the ABC transporter superfamily composed of 48 transporters, which have been extensively studied for their role in cancer multidrug resistance and, more recently, in tumorigenesis. ABCB5 has been identified as a marker of skin progenitor cells, melanoma, and limbal stem cells. It has also been associated with multidrug resistance in several cancers. The unique feature of ABCB5 is that it exists as a full transporter (ABCB5FL) and as a half transporter (ABCB5β). Several studies have shown that the ABCB5β homodimer does not confer multidrug resistance, in contrast to ABCB5FL. In this study, using three complementary techniques, nanoluciferase-based bioluminescence resonance energy transfer (NanoBRET), co-immunoprecipitation, and a proximity ligation assay, we identified two novel heterodimers in melanoma: ABCB5β/B6 and ABCB5β/B9. These results represent an important step forward in untangling the functional role of ABCB5β in melanocytes and melanoma.Competing Interest StatementThe authors have declared no competing interest.