TY - JOUR T1 - Elucidation of Global Trends in the Effects of VX-661 and VX-445 on the Expression of Clinical CFTR Variants JF - bioRxiv DO - 10.1101/2022.10.14.512300 SP - 2022.10.14.512300 AU - Andrew G. McKee AU - Eli F. McDonald AU - Wesley D. Penn AU - Charles P. Kuntz AU - Karen Noguera AU - Laura M. Chamness AU - Francis J. Roushar AU - Jens Meiler AU - Kathryn E. Oliver AU - Lars Plate AU - Jonathan P. Schlebach Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/10/25/2022.10.14.512300.abstract N2 - Cystic fibrosis (CF) is a chronic genetic disease caused by mutations that compromise the expression and/ or function of the cystic fibrosis transmembrane conductance regulator chloride channel (CFTR). Most people with CF harbor a common misfolded CFTR variant (ΔF508), which can be rescued by combination therapies containing “corrector” compounds that restore its expression. Nevertheless, there are over 400 other CF variants that differ in their sensitivity to correctors for reasons that remain unclear. In this work, we utilize deep mutational scanning to quantitatively compare the effects of two FDA-approved correctors on the plasma membrane expression of 129 known CF variants, including 45 that are currently unclassified. Across 67 variants with attenuated expression, we find that VX-661-sensitive variants generally exhibit intermediate expression and feature mutations near its binding pocket in transmembrane domains (TMDs) 1, 2, 3, and 6. VX-445 also primarily rescues variants with intermediate expression but is instead uniquely effective towards mutations near its binding pocket in TMDs 10 & 11. Structural calculations suggest corrector binding provides similar stabilization to both sensitive and insensitive variants. These findings collectively suggest the mutation-specific effects of these compounds depend on the degree of variant destabilization and/ or the timing of cotranslational folding defects. Combining these correctors synergistically rescues variants with deficient and intermediate expression alike, presumably by doubling the total binding energy and suppressing defects at different stages of translation. These results provide an unprecedented overview of the properties of rare CFTR variants and establish new tools for CF pharmacology.Competing Interest StatementThe authors have declared no competing interest. ER -