PT  - JOURNAL ARTICLE
AU  - Juechun Tang
AU  - Mark P. Brynildsen
TI  - Genome-wide mapping of fluoroquinolone-stabilized DNA gyrase cleavage sites displays drug specific effects that correlate with bacterial persistence
AID  - 10.1101/2022.10.27.514060
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.10.27.514060
4099  - http://biorxiv.org/content/early/2022/10/28/2022.10.27.514060.short
4100  - http://biorxiv.org/content/early/2022/10/28/2022.10.27.514060.full
AB  - Persisters are rare phenotypic variants that are suspected to be culprits of recurrent infections. Fluoroquinolones (FQs) are a class of antibiotic that facilitate DNA damage by stabilizing type II topoisomerases when they are in a complex with cleaved DNA. In Escherichia coli, DNA gyrase is the primary FQ target, and previous work has demonstrated that persisters are not spared from FQ-induced DNA damage. Since DNA gyrase cleavage sites (GCSs) largely govern the sites of DNA damage from FQ treatment, we hypothesized that GCS characteristics (e.g., number, strength, location) may influence persistence. To test this hypothesis, we measured genome-wide GCS distributions after treatment with a panel of FQs. We found drug-specific effects on the GCS distribution and discovered a strong negative correlation between the cumulative cleavage strength across the chromosome and FQ persister levels. Further experiments and analyses suggested that persistence was not governed by cleavage to individual sites, but rather survival was a function of the cumulative GCS distribution. Together, these findings demonstrate FQ-specific differences in GCS distribution that correlate with persister levels and suggest that FQs that better stabilize DNA gyrase in cleaved complexes with DNA will lead to lower levels of persistence.Competing Interest StatementThe authors have declared no competing interest.