RT Journal Article SR Electronic T1 Sequence-based Optimized Chaos Game Representation and Deep Learning for Peptide/Protein Classification JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.09.10.507145 DO 10.1101/2022.09.10.507145 A1 Huang, Beibei A1 Zhang, Eric A1 Chaudhari, Rajan A1 Gimperlein, Heiko YR 2022 UL http://biorxiv.org/content/early/2022/10/29/2022.09.10.507145.abstract AB As an effective graphical representation method for 1D sequence (e.g., text), Chaos Game Representation (CGR) has been frequently combined with deep learning (DL) for biological analysis. In this study, we developed a unique approach to encode peptide/protein sequences into CGR images for classification. To this end, we designed a novel energy function and enhanced the encoder quality by constructing a Supervised Autoencoders (SAE) neural network. CGR was used to represent the amino acid sequences and such representation was optimized based on the latent variables with SAE. To assess the effectiveness of our new representation scheme, we further employed convolutional neural network (CNN) to build models to study hemolytic/non-hemolytic peptides and the susceptibility/resistance of HIV protease mutants to approved drugs. Comparisons were also conducted with other published methods, and our approach demonstrated superior performance.Supplementary information available onlineCompeting Interest StatementThe authors have declared no competing interest.