RT Journal Article SR Electronic T1 Biochemical characterization and NMR study of a PET-hydrolyzing cutinase from Fusarium solani pisi JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.11.01.514593 DO 10.1101/2022.11.01.514593 A1 Kristina Naasen Hellesnes A1 Shunmathi Vijayaraj A1 Peter Fojan A1 Evamaria Petersen A1 Gaston Courtade YR 2022 UL http://biorxiv.org/content/early/2022/11/01/2022.11.01.514593.abstract AB In recent years, the drawbacks of plastics have become evident, with plastic pollution becoming a major environmental issue. There is an urgent need to find solutions to efficiently handle plastic waste by using novel recycling methods. Biocatalytic recycling of plastics by using enzyme-catalyzed hydrolysis is one such solution that has gained interest, in particular for recycling polyethylene terephthalate (PET). To provide insights on PET hydrolysis by cutinases, we have here characterized the kinetics of a PET-hydrolyzing cutinase from Fusarium solani pisi (FsC) at different pH values, mapped the interaction between FsC and the PET analog BHET by using NMR spectroscopy, and monitored product release directly and in real time by using time-resolved NMR experiments. We found that primarily aliphatic side chains around the active site participate in the interaction with BHET, and that pH conditions can be used to tune the relative amounts of degradation products. Moreover, we propose that the low catalytic performance of FsC on PET is caused by poor substrate binding combined with product inhibition by MHET. Overall, our results provide insights on obstacles that preclude efficient PET hydrolysis by FsC and suggest future approaches for overcoming these obstacles and generating efficient PET-hydrolyzing enzymes.Competing Interest StatementThe authors have declared no competing interest.