RT Journal Article
SR Electronic
T1 Human Nucleolar Protein 7 (NOL7) is required for pre-rRNA transcription and pre-18S rRNA processing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.08.515626
DO 10.1101/2022.11.08.515626
A1 Mason A. McCool
A1 Carson J. Bryant
A1 Hannah Huang
A1 Lisa M. Ogawa
A1 Katherine I. Farley-Barnes
A1 Samuel B. Sondalle
A1 Laura Abriola
A1 Yulia V. Surovtseva
A1 Susan J. Baserga
YR 2022
UL http://biorxiv.org/content/early/2022/11/08/2022.11.08.515626.abstract
AB The main components of the essential cellular process of eukaryotic ribosome biogenesis are highly conserved from yeast to humans. Among these, the transcription-U3 Associated Proteins (t-UTPs) are a small subunit processome subcomplex that coordinate the first two steps of ribosome biogenesis in transcription and pre-18S processing. While we have identified the human counterparts of most of the yeast Utps, the homologs of yeast Utp9 and Bud21 (Utp16) have remained elusive. In this study, we find NOL7 is the likely ortholog of Bud21. Previously described as a tumor suppressor through regulation of antiangiogenic transcripts, we now show that NOL7 is required for early pre-rRNA stability and pre-18S processing in human cells. These roles lead to decreased protein synthesis, induction of the nucleolar stress response, and defects in cell cycle progression upon NOL7 depletion. Beyond Bud21’s nonessential role in yeast, we establish human NOL7 as an essential UTP that is necessary for both pre-rRNA transcription and processing.Competing Interest StatementThe authors have declared no competing interest.