RT Journal Article
SR Electronic
T1 Identification of leaf rust resistance loci in a geographically diverse panel of wheat using genome-wide association analysis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.10.515255
DO 10.1101/2022.11.10.515255
A1 Kaur, Shivreet
A1 Gill, Harsimardeep
A1 Breiland, Matthew
A1 Kolmer, James
A1 Gupta, Rajeev
A1 Sehgal, Sunish
A1 Gill, Upinder
YR 2022
UL http://biorxiv.org/content/early/2022/11/11/2022.11.10.515255.abstract
AB Leaf rust, caused by Puccinia triticina (Pt) is among the most devastating diseases posing a significant threat to global wheat production. The continuously evolving virulent Pt races in North America calls for exploring new sources of leaf rust resistance. A diversity panel of 365 bread wheat accessions selected from a worldwide population of landraces and cultivars was evaluated at the seedling stage against four Pt races (TDBJQ, TBBGS, MNPSD and, TNBJS). A wide distribution of seedling responses against the four Pt races was observed. Majority of the genotypes displayed a susceptible response with only 28 (9.8%), 59 (13.5%), 45 (12.5%), and 29 (8.1%) wheat accessions exhibiting a highly resistant response to TDBJQ, TBBGS, MNPSD and, TNBJS, respectively. Further, we conducted a high-resolution multi-locus genome-wide association study (GWAS) using a set of 302,524 high-quality single nucleotide polymorphisms (SNPs). The GWAS analysis identified 27 marker-trait associations (MTAs) for leaf rust resistance on different wheat chromosomes of which 20 MTAs were found in the vicinity of known Lr genes, MTAs, or quantitative traits loci (QTLs) identified in previous studies. The remaining seven significant MTAs identified represent genomic regions that harbor potentially novel genes for leaf rust resistance. Furthermore, the candidate gene analysis for the significant MTAs identified various genes of interest that may be involved in disease resistance. The identified resistant lines and SNPs linked to the QTLs in this study will serve as valuable resources in wheat rust resistance breeding programs.Competing Interest StatementThe authors have declared no competing interest.