PT  - JOURNAL ARTICLE
AU  - Sudhir K. Shukla
AU  - T. Subba Rao
TI  - An Improved Crystal Violet Assay for Biofilm Quantification in 96-Well Microtitre Plate
AID  - 10.1101/100214
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 100214
4099  - http://biorxiv.org/content/early/2017/01/13/100214.short
4100  - http://biorxiv.org/content/early/2017/01/13/100214.full
AB  - Microplates are essential tools for biofilm research since it allows high throughput screening of biofilm forming strains or in the assay of anti-biofilm drugs. However, 96 well microtitre plate based assays share the issue of “edge effect”. The primary cause of the “edge effect” phenomenon is evaporation. As edge effect causes a significant increase in plate rejection rate by introducing experimental error, we improvised the classical crystal violet assay to reduce water loss from the peripheral wells. The improvised method showed a significant reduction in edge effect and minimised error in crystal violet assay