PT  - JOURNAL ARTICLE
AU  - Farmer, Rohit
AU  - Apps, Richard
AU  - Quiel, Juan
AU  - Sellers, Brian A.
AU  - Cheung, Foo
AU  - Chen, Jinguo
AU  - Mukherjee, Amrita
AU  - McGuire, Peter J.
AU  - Tsang, John S.
TI  - Multiparameter stimulation mapping of signaling states in single pediatric immune cells reveals heightened tonic activation during puberty
AID  - 10.1101/2022.11.14.516371
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.11.14.516371
4099  - http://biorxiv.org/content/early/2022/11/16/2022.11.14.516371.short
4100  - http://biorxiv.org/content/early/2022/11/16/2022.11.14.516371.full
AB  - Cellular stimulation via factors such as cytokines followed by multiparameter single-cell measurements is a powerful approach to interrogate cellular functions. However, transforming such high-dimensional data into biological insights presents unique challenges, particularly given the extensive response heterogeneity among single cells, such as the presence of bimodal responding versus non-responding subpopulations upon stimulation. Here we present an unsupervised high-dimensional approach for analyzing stimulation responses at the single cell level (HDStIM) and apply it to evaluate how pediatric development may shape peripheral immune cell signaling states and responsiveness to stimulations in 42 subjects (age: 2 - 16). We show that in comparison to the conventional approach of assessing one marker at a time by averaging across single cells, HDStIM can effectively learn, in an unsupervised fashion, the multi-parameter signature of responding versus non-responding cells to accurately quantify responses within cell populations. HDStIM reveals that the extent of pre-stimulation/baseline activation of interferon-related and TCR signaling molecules in myeloid and T cells, respectively, increases during puberty. This suggests that puberty is marked by a heightened “tonic” activation state in these cells, perhaps to strengthen defense against pathogens during this period of human development.Competing Interest StatementThe authors have declared no competing interest.