PT  - JOURNAL ARTICLE
AU  - Gema I Vera Gonzalez
AU  - Phatsimo O Kgwarae
AU  - Simon R Schultz
TI  - Two-Photon Targeted, Quad Whole-Cell Patch Clamping Robot
AID  - 10.1101/2022.11.14.516499
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.11.14.516499
4099  - http://biorxiv.org/content/early/2022/11/16/2022.11.14.516499.short
4100  - http://biorxiv.org/content/early/2022/11/16/2022.11.14.516499.full
AB  - We present an automated quad-channel patch-clamp technology platform for ex vivo brain slice electrophysiology, capable of both blind and two-photon targeted robotically automated patching. The robot scales up the patch clamp single cell recording technique to four simultaneous channels, with seal success rates for two-photon targeted and blind modes of 54% and 67% respectively. In 50% of targeted trials (where specific cells were required), at least 2 simultaneous recordings were obtained. For blind mode, most trials yielded dual or triple recordings. This robot, a milestone on the path to a true in vivo robotic multi-patching technology platform, will allow numerous studies into the function and connectivity patterns of both primary and secondary cell types.Competing Interest StatementThe authors have declared no competing interest.