RT Journal Article
SR Electronic
T1 Pyruvate-conjugation of PEGylated liposomes effectively enhances their uptake in retinal photoreceptors
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.18.517040
DO 10.1101/2022.11.18.517040
A1 Gustav Christensen
A1 Yiyi Chen
A1 Dileep Urimi
A1 Nicolaas Schipper
A1 François Paquet-Durand
YR 2022
UL http://biorxiv.org/content/early/2022/11/18/2022.11.18.517040.abstract
AB Despite several promising candidates there is a paucity of drug treatments available for patients suffering from retinal diseases. An important reason for this is the lack of suitable delivery systems that can achieve sufficiently high drug uptake in the retina and its photoreceptors. A promising and versatile method for drug delivery to specific cell types involves liposomes, surface-coated with substrates for transporter proteins highly expressed on the target cell.We identified strong lactate transporter (monocarboxylate transporter, MCT) expression on photoreceptors as a potential target for drug delivery vehicles. To evaluate MCT suitability for drug targeting, we used PEG-coated liposomes and conjugated these with different monocarboxylates, including lactate, pyruvate, and cysteine. Monocarboxylate-conjugated dye-loaded liposomes were tested on both human-derived cell-lines and murine retinal explant cultures. We found that liposomes conjugated with pyruvate consistently displayed higher cell uptake than unconjugated liposomes or liposomes conjugated with lactate or cysteine. Pharmacological inhibition of MCT1 and MCT2 reduced internalization, suggesting an MCT-mediated uptake mechanism. Pyruvate-conjugated liposomes loaded with the drug candidates CN03 and CN04 reduced photoreceptor cell death in murine rd1 and rd10 retinal degeneration models.Overall, this study proposes pyruvate-conjugated liposomes as a vehicle for drug delivery specifically to photoreceptors. Notably, in retinal degeneration models, free drug solutions could not achieve the same therapeutic effect. Our study thus highlights pyruvate-conjugated liposomes as a promising system for drug delivery to retinal photoreceptors, as well as other neuronal cell types displaying high expression of MCT-type proteins.Competing Interest StatementThe authors have declared no competing interest.