PT  - JOURNAL ARTICLE
AU  - Chenyang Zhao
AU  - Yuqing Liu
AU  - Youhe Gao
TI  - Urine proteomic analysis of the rat startle model
AID  - 10.1101/2022.11.19.517176
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.11.19.517176
4099  - http://biorxiv.org/content/early/2022/11/19/2022.11.19.517176.short
4100  - http://biorxiv.org/content/early/2022/11/19/2022.11.19.517176.full
AB  - Urine proteomics was applied to explore whether the effect of startle can be detected in urine. A combination of natural enemy odor and sound stimulation was used to establish the rat startle model. Urine samples were collected before and after startle, and urine proteomes before-after startle of each rat were compared individually. Regulatory subunits of glutamate-cysteine ligase was identified as the sole differential protein among all five startled rats. To our surprise, its functional partner catalytic subunits of glutamate-cysteine ligase was also identified in four out of five rats as differential protein. When comparing before-after startle as two groups, 22 differential proteins were identified which represent biological pathways including neurotransmitter transport and glucose transmembrane transport.Competing Interest StatementThe authors have declared no competing interest.