RT Journal Article
SR Electronic
T1 Urine proteomic analysis of the rat startle model
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.19.517176
DO 10.1101/2022.11.19.517176
A1 Chenyang Zhao
A1 Yuqing Liu
A1 Youhe Gao
YR 2022
UL http://biorxiv.org/content/early/2022/11/19/2022.11.19.517176.abstract
AB Urine proteomics was applied to explore whether the effect of startle can be detected in urine. A combination of natural enemy odor and sound stimulation was used to establish the rat startle model. Urine samples were collected before and after startle, and urine proteomes before-after startle of each rat were compared individually. Regulatory subunits of glutamate-cysteine ligase was identified as the sole differential protein among all five startled rats. To our surprise, its functional partner catalytic subunits of glutamate-cysteine ligase was also identified in four out of five rats as differential protein. When comparing before-after startle as two groups, 22 differential proteins were identified which represent biological pathways including neurotransmitter transport and glucose transmembrane transport.Competing Interest StatementThe authors have declared no competing interest.