PT  - JOURNAL ARTICLE
AU  - Jose Ricardo Morales Poole
AU  - Sheng Feng Huang
AU  - AnLong Xu
AU  - Pontarotti Pierre
TI  - The RAG transposon is active through the deuterostome evolution and domesticated in jawed vertebrates
AID  - 10.1101/100735
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 100735
4099  - http://biorxiv.org/content/early/2017/01/16/100735.short
4100  - http://biorxiv.org/content/early/2017/01/16/100735.full
AB  - RAG1 and RAG2 are essential subunits of the V(D)J recombinase required for the generation of the variability of antibodies and T-cell receptors in jawed vertebrates. It was demonstrated that the amphioxus homologue of RAG1-RAG2 is encoded in an active transposon, belonging to transposase DDE superfamily. We show here that the RAG transposon has been active through the deuterostome evolution and is still active in several lineages. The RAG transposon corresponds to several families present in deuterostomes. RAG1-RAG2 V(D)J recombinase evolved from one of them, partially due to the new ability of the transposon to interact with the cellular reparation machinery. Considering the fact that the RAG transposon survived millions of years in many different lineages, in multiple copies and that DDE transposases evolved many times their association with proteins involved in repair mechanisms, we propose that the apparition of V(D)J recombination machinery could be a predictable genetic event.