TY - JOUR T1 - In-depth characterization of the Syrian hamster as translational model for COVID-19 in humans JF - bioRxiv DO - 10.1101/2022.11.22.517339 SP - 2022.11.22.517339 AU - Martina Castellan AU - Gianpiero Zamperin AU - Giulia Franzoni AU - Greta Foiani AU - Maira Zorzan AU - Petra Drzewnioková AU - Marzia Mancin AU - Irene Brian AU - Alessio Bortolami AU - Matteo Pagliari AU - Annalisa Oggiano AU - Marta Vascellari AU - Valentina Panzarin AU - Sergio Crovella AU - Isabella Monne AU - Calogero Terregino AU - Paola De Benedictis AU - Stefania Leopardi Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/11/22/2022.11.22.517339.abstract N2 - The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the importance of having proper tools and models to study the pathophysiology of emerging infectious diseases to test therapeutic protocols, assess changes in viral phenotype and evaluate the effect of viral evolution. This study provides a comprehensive characterization of the Syrian hamster (Mesocricetus auratus) as an animal model for SARS-CoV-2 infection, using different approaches (description of clinical signs, viral replication, receptor profiling and host immune response) and targeting four different organs (lungs, intestine, brain and PBMCs). Our data showed that both male and female hamsters are susceptible to the infection and develop a disease similar to the one observed in patients with COVID-19, including moderate to severe pulmonary lesions, inflammation and recruitment of the immune system in lungs and at systemic level. However, all animals recovered within 14 days without developing the severe pathology seen in humans, and none of them died. We found faint evidence for intestinal and neurological tropism associated with absence of lesions and a minimal host response in intestines and brains, highlighting another crucial difference with the multi-organ impairment of severe COVID-19. When comparing male and female hamsters, it was observed that males sustained higher viral shedding and replication in lungs, suffered from more severe symptoms and histopathological lesions and triggered higher pulmonary inflammation. Overall, these data confirm the Syrian hamster as being a suitable model for mild-moderate COVID-19 and reflect sex-related differences in the response against the virus observed in humans.Competing Interest StatementThe authors have declared no competing interest. ER -