RT Journal Article
SR Electronic
T1 Investigating the potential of packed cell volume for deducing hemoglobin: Cholistani camels in perspective
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.15.516630
DO 10.1101/2022.11.15.516630
A1 Umer Farooq
A1 Musadiq Idris
A1 Nouman Sajjad
A1 Mushtaq Hussain Lashari
A1 Shahbaz Ahmad
A1 Zia Ur Rehman
A1 Haroon Rashid
A1 Aisha Mahmood
A1 Sajid Hameed
YR 2022
UL http://biorxiv.org/content/early/2022/11/23/2022.11.15.516630.abstract
AB In human medical practice, a hematological rule of three has been validated for healthy human populations. One such formula is estimating hemoglobin (Hb) levels as 1/3rd of Packed Cell Volume (PCV). However, no such hematological formulae have been devised and validated for veterinary medical practice. The present study was devised with an aim to evaluate the relationship between hemoglobin (Hb) concentration and Packed Cell Volume (PCV) in camels (n=215) being reared under pastoralism, and to devise a simple pen-side hematological formula for estimation of Hb from PCV. The PCV was determined through microhematocrit method whereas Hb estimation by cyanmethaemoglobin method (HbD). The Hb was also calculated as 1/3rd of PCV and was dubbed as calculated Hb (HbC). Overall HbD and HbC were significantly (P≥0.05) different. Similar results were attained for all study groups i.e. males (n=94) and females (n=121), and young (n=85) and adult (n=130) camels. The corrected Hb (CHb) was deduced through regression prediction equation attained from linear regression model. Scatter-plots were drawn, linear regression was carried out, and Bland Altman chart was built for agreement of both methods of Hb estimation. A non-significant (P≥0.05) difference was noticed between HbD and CHb. Bland Altman chart revealed good agreement between HbD and CHb and there was no proportional bias on the distribution of data around the mean difference line (Mean= 0.1436, 95% CI= 3.00, −2.72). We accordingly recommend a simplified pen-side hematological formula for deducing Hb concentration from PCV viz. Hb concentration (g/dL)= 0.18(PCV)+5.4 for all age and gender groups of camels instead of its calculation as one-third of PCV.Competing Interest StatementThe authors have declared no competing interest.