RT Journal Article SR Electronic T1 Transgenerational Effects of Early-Life Stress on Anxiety in Zebrafish (Danio rerio) JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.11.22.517541 DO 10.1101/2022.11.22.517541 A1 Fontana, Barbara D. A1 Alnassar, Nancy A1 Parker, Matthew O. YR 2022 UL http://biorxiv.org/content/early/2022/11/23/2022.11.22.517541.abstract AB Early-life adversity impacts on anxiety-related behaviors in adulthood. The effects of such adversity not only affects the animal itself, but can be passed on transgenerationally. Pervasive effects of experimentally-induced early-life stress (ELS) have been documented in adult zebrafish but it is not clear if this can be passed on via the germline. Here, we investigated the effects of ELS across three generations, by analyzing the responses of adult animals exposed to ELS in two different anxiety-related tasks, as well as in social behavior, memory, and cognition. Animals exposed to ELS (at 7 days-post-fertilization) showed a marked attenuation of specific anxiety-related behaviors (F0) when adults, and these alterations were maintained across two subsequent generations (F1 and F2). These findings suggest that zebrafish may be a useful model organism to study the transgenerational effects of ELS, and how this pertains to (for example) neuropsychiatric disorders. In addition, our data may naturally provoke questions regarding consideration of the environment of laboratory-housed zebrafish at early developmental stages. In particular, more work may be necessary to determine how different environmental stressors could affect data variability across laboratories.Summary of the ELS effects in zebrafish anxiety-like behavior across multiple generations.Competing Interest StatementThe authors have declared no competing interest.