RT Journal Article
SR Electronic
T1 Organism-wide secretome mapping uncovers pathways of tissue crosstalk in exercise
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.21.517385
DO 10.1101/2022.11.21.517385
A1 Wei Wei
A1 Nicholas M. Riley
A1 Xuchao Lyu
A1 Xiaotao Shen
A1 Jing Guo
A1 Meng Zhao
A1 Maria Dolores Moya-Garzon
A1 Himanish Basu
A1 Alan Tung
A1 Veronica L. Li
A1 Wentao Huang
A1 Katrin J. Svensson
A1 Michael P. Snyder
A1 Carolyn R. Bertozzi
A1 Jonathan Z. Long
YR 2022
UL http://biorxiv.org/content/early/2022/11/23/2022.11.21.517385.abstract
AB There has been growing interest in identifying blood-borne factors that mediate tissue crosstalk and function as molecular effectors of physical activity. Although past studies have focused on an individual molecule or cell type, the organism-wide secretome response to physical activity has not been evaluated. Here, we use a cell type-specific proteomic approach to generate a 21-cell type, 10-tissue map of exercise-regulated secretomes in mice. Our dataset identifies >200 exercise-regulated cell type-secreted protein pairs, the majority of which have not been previously reported. Pdgfra-cre-labeled secretomes were the most responsive to exercise training. Elevated lactate levels can directly regulate protein secretion. Lastly, we establish an anti-obesity and anti-diabetic role for a proteoform of an intracellular carboxylesterase whose secretion from the liver is induced by exercise training. Together, our data uncover the dynamic remodeling of cell and tissue crosstalk by physical activity.Competing Interest StatementThe authors have declared no competing interest.