PT - JOURNAL ARTICLE AU - Zheng, Zhihao AU - Own, Christopher S. AU - Wanner, Adrian A. AU - Koene, Randal A. AU - Hammerschmith, Eric W. AU - Silversmith, William M. AU - Kemnitz, Nico AU - Tank, David W. AU - Seung, H. Sebastian TI - Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy AID - 10.1101/2022.11.23.517701 DP - 2022 Jan 01 TA - bioRxiv PG - 2022.11.23.517701 4099 - http://biorxiv.org/content/early/2022/11/24/2022.11.23.517701.short 4100 - http://biorxiv.org/content/early/2022/11/24/2022.11.23.517701.full AB - We have achieved a three fold increase in the speed of transmission electron microscopy by using a beam deflecting mechanism to enable highly efficient acquisition of multiple image tiles for each motion of the mechanical stage. For millimeter-scale areas, the duty cycle of imaging doubles and exceeds 30%, yielding a net average imaging rate of 0.3 gigapixels per second.Competing Interest StatementN.K. and H.S.S. disclose financial interests in Zetta AI LLC. C.S.O. and R.A.K. disclose financial interests in Voxa. A.A.W. is a founder and owner of ariadne.ai ag (Switzerland).