TY - JOUR T1 - Genetic ablation of a female specific Apetala 2 transcription factor blocks oocyst shedding in <em>Cryptosporidium parvum</em> JF - bioRxiv DO - 10.1101/2022.11.23.517783 SP - 2022.11.23.517783 AU - Jayesh Tandel AU - Katelyn A. Walzer AU - Jessica H. Byerly AU - Brittain Pinkston AU - Daniel P. Beiting AU - Boris Striepen Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/11/24/2022.11.23.517783.abstract N2 - The apicomplexan parasite Cryptosporidium is a leading global cause of diarrheal disease, and the infection poses a particularly grave threat to young children and those with weakened immune function. Infection occurs by ingestion of meiotic spores called oocysts, and transmission relies on fecal shedding of new oocysts. The entire lifecycle thus occurs in a single host and features asexual as well as sexual forms of replication. Here we identify and locus tag two Apetala 2-type (AP2) transcription factors and demonstrate that they are exclusively expressed in male and female gametes, respectively. To enable functional studies of essential genes in C. parvum we develop and validate a small molecule inducible gene excision system, which we apply to the female factor AP2-F to achieve conditional gene knock out. Analyzing this mutant, we find the factor to be dispensable for asexual growth and early female fate determination in vitro, but to be required for oocyst shedding in infected animals in vivo.Transcriptional analyses conducted in the presence or absence of AP2-F revealed that the factor controls the transcription of genes encoding crystalloid body proteins, which are exclusively expressed in female gametes. In C. parvum, the organelle is restricted to sporozoites, and its loss in other apicomplexan parasites leads to blocked transmission. Overall, our development of conditional gene ablation in C. parvum provides a robust method for genetic analysis in this parasite that enabled us to identify AP2-F as an essential regulator of transcription required for oocyst shedding and transmission. ER -