RT Journal Article
SR Electronic
T1 A non-invasive, fast on/off “Odourgenetic” Method to Manipulate Physiology
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.25.517891
DO 10.1101/2022.11.25.517891
A1 Shanchun Su
A1 Yanqiong Wu
A1 Xueqin Xu
A1 Xincai Hao
A1 Wei Lu
A1 Xiaohui Li
A1 Linhan Wang
A1 Wei Tian
A1 Yan Gao
A1 Gang Cao
A1 Changbin Ke
YR 2022
UL http://biorxiv.org/content/early/2022/11/25/2022.11.25.517891.abstract
AB Manipulating molecular processes governing physiological functions has significant potential for clinical therapeutics and is an important approach to elucidate the cellular basis of physiological functions. Here, we designed a Odourgenetic system co-expressed Drosophila odorant receptor system (DORs) consisting of OR35a and OR83b, which were exclusively activated by their odor ligand, 2-pentanone. Applying 2-pentanone to DOR-expressing cells or tissues induced calcium influx and membrane depolarization. By inhalation of 2-pentanone, we successfully applied DORs to manipulate behaviour, control insulin secretion and regulate blood glucose and manipulate muscle contraction and associated limb movement. Because 2-pentanone rapidly enters the blood upon inhalation and leaves the body by exhalation, this odorant can be used with DORs to manipulate cellular function, and the manipulation can be terminated at any time. Such feature approach significantly improves the safety and controllability of DORs used in the clinic. Thus, the present study developed a non-invasive, controllable, fast on/off method to manipulate cellular activity and behaviour on a time scale of minutes.Competing Interest StatementThe authors have declared no competing interest.