PT  - JOURNAL ARTICLE
AU  - Kadobianskyi, Mykola
AU  - Schulze, Lisanne
AU  - Schuelke, Markus
AU  - Judkewitz, Benjamin
TI  - Hybrid genome assembly and annotation of <em>Danionella translucida</em>
AID  - 10.1101/539692
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 539692
4099  - http://biorxiv.org/content/early/2019/03/18/539692.short
4100  - http://biorxiv.org/content/early/2019/03/18/539692.full
AB  - Studying neuronal circuits at cellular resolution is very challenging in vertebrates due to the size and optical turbidity of their brains. Danionella translucida, a close relative of zebrafish, was recently introduced as a model organism for investigating neural network interactions in adult individuals. Danionella remains transparent throughout its life, has the smallest known vertebrate brain and possesses a rich repertoire of complex behaviours. Here we sequenced, assembled and annotated the Danionella translucida genome employing a hybrid Illumina/ Nanopore read library as well as RNA-seq of embryonic, larval and adult mRNA. We achieved high assembly continuity using low-coverage long-read data and annotated a large fraction of the transcriptome. This dataset will pave the way for molecular research and targeted genetic manipulation of this novel model organism.