PT  - JOURNAL ARTICLE
AU  - Alexandra Sneddon
AU  - Agin Ravindran
AU  - Nadine Hein
AU  - Nikolay Shirokikh
AU  - Eduardo Eyras
TI  - Real-time biochemical-free targeted sequencing of RNA species with RISER
AID  - 10.1101/2022.11.29.518281
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.11.29.518281
4099  - http://biorxiv.org/content/early/2022/11/29/2022.11.29.518281.short
4100  - http://biorxiv.org/content/early/2022/11/29/2022.11.29.518281.full
AB  - We present the first biochemical-free technology for Real-time In Silico Enrichment of RNA species (RISER). RISER classifies RNA species from direct RNA nanopore signals, without the need for basecalling or a reference, and communicates with the sequencing hardware in real-time to enact in silico targeted RNA sequencing. We illustrate RISER for the enrichment and depletion of coding and non-coding RNA, demonstrating a 3.4-3.6x enrichment and 6.2-6.7x depletion of non-coding RNA in live sequencing experiments.Competing Interest StatementA.S. has received funds from Oxford Nanopore Technologies for travel and accommodation to speak at the Oxford Nanopore Technologies conference London Calling 2022. The authors declare no other competing interests.