RT Journal Article
SR Electronic
T1 Real-time biochemical-free targeted sequencing of RNA species with RISER
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.29.518281
DO 10.1101/2022.11.29.518281
A1 Alexandra Sneddon
A1 Agin Ravindran
A1 Nadine Hein
A1 Nikolay Shirokikh
A1 Eduardo Eyras
YR 2022
UL http://biorxiv.org/content/early/2022/11/29/2022.11.29.518281.abstract
AB We present the first biochemical-free technology for Real-time In Silico Enrichment of RNA species (RISER). RISER classifies RNA species from direct RNA nanopore signals, without the need for basecalling or a reference, and communicates with the sequencing hardware in real-time to enact in silico targeted RNA sequencing. We illustrate RISER for the enrichment and depletion of coding and non-coding RNA, demonstrating a 3.4-3.6x enrichment and 6.2-6.7x depletion of non-coding RNA in live sequencing experiments.Competing Interest StatementA.S. has received funds from Oxford Nanopore Technologies for travel and accommodation to speak at the Oxford Nanopore Technologies conference London Calling 2022. The authors declare no other competing interests.