RT Journal Article
SR Electronic
T1 Time-Aligned Hourglass Gastrulation Models in Rabbit and Mouse
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.13.516304
DO 10.1101/2022.11.13.516304
A1 Y Mayshar
A1 O Raz
A1 S Cheng
A1 R Ben-Yair
A1 R Hadas
A1 N Reines
A1 M Mittnenzweig
A1 O Ben-Kiki
A1 A Lifshitz
A1 A Tanay
A1 Y Stelzer
YR 2022
UL http://biorxiv.org/content/early/2022/11/30/2022.11.13.516304.abstract
AB The hourglass model describes the convergence of species within the same phylum to a similar body plan during development, yet the molecular mechanisms underlying this phenomenon in mammals remain poorly described. Here, we compare rabbit and mouse time-resolved differentiation trajectories to revisit this model at single cell resolution. We modeled gastrulation dynamics using hundreds of embryos sampled between gestation days 6.0-8.5, and compare the species using a new framework for time-resolved single-cell differentiation-flows analysis. We find convergence toward similar cell state compositions at E7.5, underlied by quantitatively conserved expression of 76 transcription factors, despite divergence in surrounding trophoblast and hypoblast signaling. However, we observed noticeable changes in specification timing of some lineages, and divergence of primordial germ cells programs, which in the rabbit do not activate mesoderm genes. Comparative analysis of temporal differentiation models provides a new basis for studying the evolution of gastrulation dynamics across mammals.Competing Interest StatementThe authors have declared no competing interest.