PT  - JOURNAL ARTICLE
AU  - Afra Sabei
AU  - Talissa Gabriele Caldas Baia
AU  - Raphaël Saffar
AU  - Juliette Martin
AU  - Elisa Frezza
TI  - Internal Normal Mode Analysis applied to RNA flexibility and conformational changes
AID  - 10.1101/2022.11.30.518608
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.11.30.518608
4099  - http://biorxiv.org/content/early/2022/12/01/2022.11.30.518608.short
4100  - http://biorxiv.org/content/early/2022/12/01/2022.11.30.518608.full
AB  - We investigated the capability of internal normal modes to reproduce RNA dynamics and predict observed RNA conformational changes, and, notably, those induced by the formation of RNA-protein and RNA-ligand complexes. Here, we extended our iNMA approach developed for proteins to study RNA molecules using a simplified representation of RNA structure and its potential energy. Three datasets were also created to investigate different aspects. Despite all the approximations, our study shows that iNMA is a suitable method to take into account RNA flexibility and describe its conformational changes opening the route to its applicability in any integrative approach where these properties are crucial.Competing Interest StatementThe authors have declared no competing interest.