RT Journal Article
SR Electronic
T1 Organization, genomic targeting and assembly of three distinct SWI/SNF chromatin remodeling complexes in <em>Arabidopsis</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.11.24.517835
DO 10.1101/2022.11.24.517835
A1 Wei Fu
A1 Yaoguang Yu
A1 Jie Shu
A1 Zewang Yu
A1 Tao Zhu
A1 Yixiong Zhong
A1 Zhihao Zhang
A1 Zhenwei Liang
A1 Yuhai Cui
A1 Chen Chen
A1 Chenlong Li
YR 2022
UL http://biorxiv.org/content/early/2022/12/07/2022.11.24.517835.abstract
AB Switch defective/sucrose non-fermentable (SWI/SNF) complexes are evolutionarily conserved multi-subunit machines that play vital roles in chromatin architecture regulation for modulating gene expression via sliding or ejection of nucleosomes in eukaryotes. In plants, perturbations of SWI/SNF subunits often result in severe developmental disorders. However, the subunit composition, pathways of assembly, and genomic targeting of the plant SWI/SNF complexes remain undefined. Here, we reveal the organization, genomic targeting and assembly of three distinct Arabidopsis SWI/SNF complexes: BRAHMA-Associated SWI/SNF complexes (BAS), SPLAYED-Associated SWI/SNF complexes (SAS) and MINUSCULE-Associated SWI/SNF complexes (MAS). We show that BAS complexes are equivalent to human ncBAF, whereas SAS and MAS complexes evolve in multiple subunits unique to plants, suggesting a plant-specific functional evolution of SWI/SNF complexes. We further demonstrate overlapping and specific genomic targeting of the three plant SWI/SNF complexes on chromatin and reveal that SAS complexes are necessary for the correct genomic localization of the BAS complexes. Finally, we define the role of core module subunit in the assembly of the plant SWI/SNF complexes and highlight that ATPase module subunit is required for global complex stability and the interaction of core module subunits in SAS and BAS complexes in Arabidopsis. Together, our work highlights the divergence of SWI/SNF chromatin remodelers during the eukaryote evolution and provides a comprehensive landscape for understanding the plant SWI/SNF complexes organization, assembly, genomic targeting, and function.One-sentence summary Comprehensively define the organization, genomic targeting and assembly of three distinct SWI/SNF chromatin remodeling complexes in ArabidopsisCompeting Interest StatementThe authors have declared no competing interest.