PT  - JOURNAL ARTICLE
AU  - Romina Piscitello-Gómez
AU  - Franz S Gruber
AU  - Abhijeet Krishna
AU  - Charlie Duclut
AU  - Carl D Modes
AU  - Marko Popović
AU  - Frank Jülicher
AU  - Natalie A Dye
AU  - Suzanne Eaton
TI  - Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in the <em>Drosophila</em> pupal wing
AID  - 10.1101/2022.12.09.519799
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.12.09.519799
4099  - http://biorxiv.org/content/early/2022/12/10/2022.12.09.519799.short
4100  - http://biorxiv.org/content/early/2022/12/10/2022.12.09.519799.full
AB  - How morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing of Drosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. We showed previously (Etournay et al., 2015) that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned. The core planar cell polarity (PCP) pathway influences morphogenetic cell movements in many other contexts, which suggests that it could globally pattern active cellular behaviors during pupal wing morphogenesis. We show here, however, that this is not the case: there is no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation, they do not affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes during Drosophila pupal wing morphogenesis are independent of one potential chemical guiding cue, core PCP.Competing Interest StatementThe authors have declared no competing interest.