TY - JOUR T1 - Anatomy of the complete mouse eye vasculature in development and pathology explored by light-sheet fluorescence microscopy JF - bioRxiv DO - 10.1101/2022.12.20.521194 SP - 2022.12.20.521194 AU - Luc Krimpenfort AU - Maria Garcia-Collado AU - Tom van Leeuwen AU - Filippo Locri AU - Anna-Liisa Luik AU - Antonio Queiro-Palou AU - Shigeaki Kanatani AU - Helder André AU - Per Uhlén AU - Lars Jakobsson Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/12/20/2022.12.20.521194.abstract N2 - Eye development and function rely on precise establishment, regression and maintenance of its many sub-vasculatures. These crucial vascular properties have been extensively investigated in eye development and disease utilizing genetic and experimental mouse models. However, due to technical limitations, individual studies have often restricted their focus to one specific sub-vasculature. Here, we apply a workflow that allows for visualisation of complete vasculatures of mouse eyes of various developmental stages. Through tissue depigmentation, immunostaining, clearing and light-sheet fluorescence microscopy (LSFM) entire vasculatures of the retina, vitreous (hyaloids) and uvea were simultaneously imaged at high resolution. In silico dissection provided detailed information on their 3D architecture and interconnections. By this method we describe remodelling of the postnatal iris vasculature following its disconnection to the feeding hyaloid vasculature. In addition, we demonstrate examples of conventional and LSFM-mediated analysis of choroidal neovascularisation after laser-induced wounding, showing added value of the presented workflow in analysis of modelled eye disease. These advancements in visualisation and analysis of the respective eye vasculatures in development and complex eye disease open for novel observations of their functional interplay at a whole-organ level.Competing Interest StatementThe authors have declared no competing interest. ER -