PT  - JOURNAL ARTICLE
AU  - Trinity McIntyre
AU  - Lalitya Andaloori
AU  - Glen Ray Hood
AU  - Jeffrey L. Feder
AU  - Daniel A. Hahn
AU  - Gregory J. Ragland
AU  - Jantina Toxopeus
TI  - Cold tolerance and diapause within and across trophic levels: endoparasitic wasps and their fly host have similar phenotypes
AID  - 10.1101/2023.01.04.522725
DP  - 2023 Jan 01
TA  - bioRxiv
PG  - 2023.01.04.522725
4099  - http://biorxiv.org/content/early/2023/01/04/2023.01.04.522725.short
4100  - http://biorxiv.org/content/early/2023/01/04/2023.01.04.522725.full
AB  - Low temperatures associated with winter can limit the survival of organisms, especially ectotherms whose body temperature is similar to their environment. Important adaptations for overwintering such as cold hardiness and diapause have been well-explored in many insect taxa. However, there is a gap in understanding how overwintering may vary among groups of species that interact closely, such as multiple parasitoid species that attack the same host insect. Our study investigated cold tolerance and diapause phenotypes in three endoparasitoid wasps of the apple maggot fly Rhagoletis pomonella (Diptera: Tephritidae): Utetes canaliculatus, Diachasma alloeum, and Diachasmimorpha mellea (Hymenoptera: Braconidae). Using a combination of respirometry and eclosion tracking, we detected diapause phenotypes in all three wasp species, remarkably similar to the fly host. Weak diapause was rare (< 5%) in all three wasp species, and while most D. mellea (93%) entered prolonged diapause under warm conditions, the majority of U. canaliculatus (92%) and D. alloeum (72%) averted diapause (non-diapause). There was limited interspecific variation in acute cold tolerance among the three wasp species: wasps and flies had similarly high survival (>87%) following exposure to extreme low temperatures (- 20°C) as long as their body fluids did not freeze. The wasp species showed little interspecific variation in survival following prolonged exposure to mild chilling of 8 or more weeks at 4°C. This study shows remarkable conservation of cold tolerance and diapause phenotypes within and across trophic levels. The interaction between diapause phenotype and cold hardiness in these parasitoids is an interesting direction for future research.The apple maggot fly and its parasitoids exhibit the same three diapause phenotypesEach parasitoid wasp species exhibits different proportions of these phenotypesUtetes canaliculatus and Diachasma alloeum are freeze-avoidant, like their host flyThese wasps and flies survive to similarly extreme low temperatures (c. -20°C)Each wasp species survives prolonged exposure to mild chilling (4°C) similarly wellCompeting Interest StatementThe authors have declared no competing interest.