@article {Maklouf2023.01.04.522727, author = {Giovanna Resk Maklouf and Gabriela Rapozo Guimar{\~a}es and Cristiane Esteves Teixeira and Marco Ant{\^o}nio Pretti and Leandro de Oliveira Santos and Nayara Gusm{\~a}o Tessarollo and Nayara Evelin Toledo and Marcelo Falchetti and Mylla M. Dimas and Alessandra Freitas Serain and Nina Carrossini Bastos and Fabiane Carvalho de Macedo and Fabiana Resende Rodrigues and Jesse Lopes da Silva and Edroaldo Lummertz da Rocha and Cl{\'a}udia Bessa Pereira Chaves and Andreia Cristina de Melo and Pedro Manoel Mendes Moraes-Vieira and Marcelo A. Mori and Mariana Boroni}, title = {A multi-tissue single-cell tumor microenvironment atlas reveals myeloid-derived cell states with significant impact on clinical outcome}, elocation-id = {2023.01.04.522727}, year = {2023}, doi = {10.1101/2023.01.04.522727}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Immunotherapies provide long-lasting responses across a wide range of cancers; however, only a fraction of patients responds to them. Multiple reasons contribute to the failure, including the existence of myeloid-derived cells (MDCs) within tumors. Due to their high plasticity, these cells display numerous cell states and, as a result, distinct pro-tumorigenic behaviors, making them interesting targets. However, the creation of cutting-edge anticancer therapies is hampered by the lack of MDC-specific markers. To fully define the MDC landscape in solid tumors, we combined single-cell RNA-Seq from 13 public datasets, including samples from seven different cancers and normal samples, yielding the largest collection of MDC subpopulations within the tumor microenvironment. We identified five major lineages subdivided into one mast cell cluster, three neutrophils, eight dendritic cells, six monocytes, and eleven macrophage states. Transcriptional profiles coupled with deconvolution estimates of cell populations in large cohorts revealed five MDC subpopulations as independent prognostic markers in different cancer types, including resident tissue interstitial macrophages and FCGR3A+ monocytes associated with an unfavorable clinical outcome in ovarian and breast cancer patients, respectively. Our work reveals that TREM2+ macrophages can be distinguished in different populations and associated with distinct prognoses. By analyzing a Brazilian cohort of ovarian cancer, we found that TREM2+ macrophages are associated with a better prognosis, indicating that their role might be dependent on the tumor niche and co-expression of immunosuppressive markers. Collectively, this atlas reveals in high-resolution the heterogeneous MDC identity as well as new avenues for understanding and manipulating their fate in cancer.One Sentence Summary Myeloid-derived cell atlas exhibit heterogeneous phenotypes in the tumor microenvironment strongly linked with clinical outcomes in cancer.Competing Interest StatementThe authors have declared no competing interest.}, URL = {https://www.biorxiv.org/content/early/2023/01/04/2023.01.04.522727}, eprint = {https://www.biorxiv.org/content/early/2023/01/04/2023.01.04.522727.full.pdf}, journal = {bioRxiv} }