RT Journal Article
SR Electronic
T1 Transcription factors mediating regulation of photosynthesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2023.01.06.522973
DO 10.1101/2023.01.06.522973
A1 Wiebke Halpape
A1 Donat Wulf
A1 Bart Verwaaijen
A1 Anna Sophie Stasche
A1 Sanja Zenker
A1 Janik Sielemann
A1 Sebastian Tschikin
A1 Prisca Viehöver
A1 Manuel Sommer
A1 Andreas P. M. Weber
A1 Carolin Delker
A1 Marion Eisenhut
A1 Andrea Bräutigam
YR 2023
UL http://biorxiv.org/content/early/2023/01/06/2023.01.06.522973.abstract
AB Photosynthesis by which plants convert carbon dioxide to sugars using the energy of light is fundamental to life as it forms the basis of nearly all food chains. Surprisingly, our knowledge about its transcriptional regulation remains incomplete. Effort for its agricultural optimization have mostly focused on post-translational regulatory processes1–3 but photosynthesis is regulated at the post-transcriptional4 and the transcriptional level5. Stacked transcription factor mutations remain photosynthetically active5,6 and additional transcription factors have been difficult to identify possibly due to redundancy6 or lethality. Using a random forest decision tree-based machine learning approach for gene regulatory network calculation7 we determined ranked candidate transcription factors and validated five out of five tested transcription factors as controlling photosynthesis in vivo. The detailed analyses of previously published and newly identified transcription factors suggest that photosynthesis is transcriptionally regulated in a partitioned, non-hierarchical, interlooped network.Competing Interest StatementThe authors have declared no competing interest.