TY - JOUR T1 - Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase JF - bioRxiv DO - 10.1101/2023.01.12.523718 SP - 2023.01.12.523718 AU - Paul Batty AU - Christoph C.H. Langer AU - Zsuzsanna Takács AU - Wen Tang AU - Claudia Blaukopf AU - Jan-Michael Peters AU - Daniel W. Gerlich Y1 - 2023/01/01 UR - http://biorxiv.org/content/early/2023/01/12/2023.01.12.523718.abstract N2 - Genetic information is stored in linear DNA molecules, which fold extensively inside cells. DNA replication along the folded template path yields two sister chromatids that initially occupy the same nuclear region in a highly intertwined arrangement. Dividing cells must disentangle and condense the sister chromatids into separate bodies such that a microtubule-based spindle can move them to opposite poles. While the spindle-mediated transport of sister chromatids has been studied in detail, the chromosome-intrinsic mechanics pre-segregating sister chromatids have remained elusive. Here, we show that human sister chromatids resolve extensively already during interphase, in a process dependent on the loop-extruding activity of cohesin, but not that of condensins. Increasing cohesin’s looping capability increases sister DNA resolution in interphase nuclei to an extent normally seen only during mitosis, despite the presence of abundant arm cohesion. That cohesin can resolve sister chromatids so extensively in the absence of mitosis-specific activities indicates that DNA loop extrusion is a generic mechanism for segregating replicated genomes, shared across different Structural Maintenance of Chromosomes (SMC) protein complexes in all kingdoms of life.Competing Interest StatementThe authors have declared no competing interest. ER -