PT - JOURNAL ARTICLE AU - Kok Lian Ho AU - Chare Li Kueh AU - Poay Ling Beh AU - Wen Siang Tan AU - David Bhella TI - Cryo-Electron Microscopy Structure of the <em>Macrobrachium rosenbergii</em> Nodavirus Capsid at 7 Angstroms Resolution AID - 10.1101/103424 DP - 2017 Jan 01 TA - bioRxiv PG - 103424 4099 - http://biorxiv.org/content/early/2017/01/26/103424.short 4100 - http://biorxiv.org/content/early/2017/01/26/103424.full AB - White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. Recombinant expression of the capsid protein of MrNV in insect cells leads to the production of VLPs closely resembling the native virus. We have investigated the structure of MrNV VLPs by cryogenic electron microscopy, determining a structure of the viral capsid at 7 angstroms resolution. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T=3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV along with the related virus of marine shrimp Penaeus vannamei nodavirus (PvNV) may represent a new genus of the Nodaviridae.Author summary Macrobrachium rosenbergii nodavirus (MrNV) is the causative agent of white tail disease (WTD) which leads to 100% mortality in shrimp-farms growing giant freshwater prawn (M. rosenbergii). MrNV is therefore a significant threat to food security and causes severe economic losses in developing countries such as Malaysia, Indonesia, Pakistan, Thailand and India. Here we have used electron microscopy to study the three-dimensional structure of MrNV, revealing that the viral capsid – the protein shell that encloses the viral genome, protecting it and transporting it from one host to the next – is differently organised to capsids produced by other viruses in the nodavirus family. The virus was found to have large blade-like spikes on its outer surface that are likely important in the early stages of infection, when the virus attaches to and enters a host cell.