RT Journal Article SR Electronic T1 A miRNA catalogue and ncRNA annotation of the short-living fish Nothobranchius furzeri JF bioRxiv FD Cold Spring Harbor Laboratory SP 103697 DO 10.1101/103697 A1 Mario Baumgart A1 Emanuel Barth A1 Aurora Savino A1 Marco Groth A1 Philipp Koch A1 Andreas Petzold A1 Ivan Arisi A1 Matthias Platzer A1 Manja Marz A1 Alessandro Cellerino YR 2017 UL http://biorxiv.org/content/early/2017/01/27/103697.abstract AB Background: The short-lived fish Nothobranchius furzeri is the shortest-lived vertebrate that can be cultured in captivity and was recently established as a model organism for aging research. Small non-coding RNAs, especially miRNAs, are implicated in age-dependent control of gene expression.Results: Here, we present a comprehensive catalogue of miRNAs and several other non-coding RNA classes (ncRNAs) for Nothobranchius furzeri. Analyzing multiple small RNA-Seq libraries, we show most of these identified miRNAs are expressed in at least one of seven Nothobranchius species. Additionally, duplication and clustering of N. furzeri miRNAs was analyzed and compared to the four fish species Danio rerio, Oryzias latipes, Gasterosteus aculeatus and Takifugu rubripes. A peculiar characteristic of N. furzeri as compared to other teleosts was a duplication of the miR-29 cluster.Conclusion: The completeness of the catalogue we provide is comparable to that of zebrafish. This catalogue represents a basis to investigate the role of miRNAs in aging and development in this species.Availability: All supplementary material can be found online at http://www.rna.uni-jena.de/en/supplements/nothobranchius-furzeri-mirnome/.