TY - JOUR T1 - Assessing assay absorption artefacts in <em>in vitro</em> cell responses to particles JF - bioRxiv DO - 10.1101/2023.01.16.524328 SP - 2023.01.16.524328 AU - Seiha Yen AU - Graeme R. Zosky AU - Yong Song Y1 - 2023/01/01 UR - http://biorxiv.org/content/early/2023/01/19/2023.01.16.524328.abstract N2 - In this study, we assessed the issue of coal particles absorbing extracellular proteins and tested the effects of different culture conditions and processing strategies to address this issue. Our data show that there is no effective strategy to solve this problem. We agree with previous reports that cytokine binding experiments should be performed to implement appropriate correction factors in order to calculate the accurate production of secreted cytokines in the supernatant of cell culture experiments. This is an underappreciated issue in many published studies on the comparative potency of particles from different sources.Competing Interest StatementThe authors have declared no competing interest. ER -