TY - JOUR T1 - Rodent ultrasonic vocal interaction resolved with millimeter precision using hybrid beamforming JF - bioRxiv DO - 10.1101/2023.01.18.524540 SP - 2023.01.18.524540 AU - M. L. Sterling AU - B. Englitz Y1 - 2023/01/01 UR - http://biorxiv.org/content/early/2023/01/20/2023.01.18.524540.abstract N2 - Ultrasonic vocalizations (USVs) fulfill an important role in communication and navigation in many species. Because of their social and affective significance, rodent USVs are increasingly used as a behavioral measure in neurodevelopmental and neurolinguistic research. Reliably attributing USVs to their emitter during close interactions has emerged as a difficult, key challenge. If addressed, all subsequent analyses gain substantial confidence.We present a hybrid ultrasonic tracking system, HyVL, that synergistically integrates a high-resolution acoustic camera with high-quality ultrasonic microphones. HyVL is the first to achieve millimeter precision (~3.4-4.8mm, 91% assigned) in localizing USVs, ~3x better than other systems, approaching the physical limits (mouse snout ~ 10mm).We analyze mouse courtship interactions and demonstrate that males and females vocalize in starkly different relative spatial positions, and that the fraction of female vocalizations has likely been overestimated previously due to imprecise localization. Further, we find that male mice vocalize more intensely when interacting with two mice, an effect mostly driven by the dominant male.HyVL substantially improves the precision with which social communication between rodents can be studied. It is also affordable, open-source, easy to set up, can be integrated with existing setups, and reduces the required number of experiments and animals.Data & Code Availability During the review process, reviewers can access all Data and Code via the link below: https://data.donders.ru.nl/loqin/reviewer-208072048/iJ4c-oRNlPIp3vArKiYQ0lAW9FipiHL8foxSzwt1FDAUpon acceptance, these materials will be made available to the public.Competing Interest StatementThe authors have declared no competing interest. ER -